Proposal for Constructing BAC Libraries for Xiphophorus Species
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چکیده
The genus Xiphophorus comprises 23 recognized (and two unnamed) species of live-bearing fishes that populate freshwater habitats on the Atlantic slopes of Mexico, Guatemala, Belize, and Honduras. Members of this genus possess a relatively low degree of post-fertilization (post-zygotic) reproductive isolation with one another. In native habitats combinations of spatial, ecological, behavioral, and anatomic factors discourage cross-species mating, such that naturally occurring interspecific Xiphophorus hybrids are virtually unknown (ROSEN 1979; ROSENTHAL et al. 2003). In the laboratory however, most members of the genus are inter-fertile with one another and produce fertile offspring, either through interspecific pairings in aquaria, or by means of artificial insemination procedures. This capability has led to the experimental crossing/backcrossing of many different species combinations and has revealed a diversity of cross-specific developmental perturbations influencing the regulation of pigmentation patterns and intensity, growth and maturation rates, behavioral patterns, DNA repair rates, and susceptibilities to spontaneous, ultraviolet radiation (UVR)-induced, and chemical mutagen-induced neoplasias. Consequently, the Xiphophorus species complex represents an easily manipulated and astoundingly diverse model system for examining the evolution of interacting gene complexes, and for dissecting the genetic bases of developmental programs, behaviors, and the molecular processes underlying neoplastic disease initiation and progression.
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تاریخ انتشار 2003